Figure 3 | Scientific Reports

Figure 3

From: Emodin ameliorates bleomycin-induced pulmonary fibrosis in rats by suppressing epithelial-mesenchymal transition and fibroblast activation

Figure 3

Emodin promotes cell apoptosis in both basal and TGF-β1-stimulated human fibroblasts.

HELFs were treated with TGF-β1 (10 ng/mL) and the indicated concentrations of emodin (30 and 60 μM) for 24 h. (a) The cells were double-stained with Annexin V-FITC and PI, and then the cellular apoptosis was determined by flow cytometry. (b) The ratio of apoptotic cells (Annexin V+ PI-, Annexin V- PI+ and Annexin V+ PI+) was statistically analyzed. (c) The protein expression of caspase-3, cleaved caspase-3, caspase-8, cleaved caspase-8, Bax and Bcl-2 was analyzed by Western blot assay. (d–f) Densitometric analysis of proteins of interest in the immunoblots using GAPDH as the internal reference. (g) The ratio of Bax/Bcl-2. Data are expressed as mean ± SD; n = 6, *P < 0.05; **P < 0.01.

Back to article page