Figure 6 | Scientific Reports

Figure 6

From: Emodin ameliorates bleomycin-induced pulmonary fibrosis in rats by suppressing epithelial-mesenchymal transition and fibroblast activation

Figure 6

Emodin represses TGF-β1 expression, EMT and fibroblast activation in vivo.

Rats were intratracheally injected with a single dose of BLM (3.0 mg/kg) and followed by emodin (20 mg/kg) or vehicle by gavage daily for 21 days. (a) Western blot was used to analyze the protein levels of TGF-β1, FSP-1, α-SMA, E-cadherin and vimentin in the lung tissues. (b) Densitometric analysis of TGF-β1, FSP-1, α-SMA, E-cadherin and vimentin in the immunoblots using GAPDH as the internal reference. (c,d) Immunohistochemical staining of vimentin- and α-SMA-positive cells in the lungs, and images at 200× magnification. (e,f) Real-time PCR was performed to detect the mRNA levels of E-cadherin and vimentin in the pulmonary tissues derived from each group. Data are expressed as mean ± SD, n = 6, *P < 0.05; **P < 0.01.

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