Figure 6 | Scientific Reports

Figure 6

From: Prolyl Hydroxylase Domain-Containing Protein 2 (Phd2) Regulates Chondrocyte Differentiation and Secondary Ossification in Mice

Figure 6

Elevated expression of chondrocyte hypertrophy marker and HIF signaling targets in SOC primary chondrocytes treated with PHD inhibitor DMOG.

Primary chondrocytes isolated from SOCs were treated with 500 μM PHD inhibitor DMOG or vehicle control. Cells were extracted for real time RT-PCR analysis. (A) mRNA levels of Col2 and Aggrecan in DMOG treated chondrocytes (normalized to vehicle control). (B) mRNA levels of hypertrophy markers, Col10 and MMP13, in DMOG treated chondrocytes. (C) mRNA levels of HIF signaling targets, Epo and Vegf, in DMOG treated chondrocytes. (D) mRNA levels of HIF signaling targets, Glut1, Pdk1, and Pgk1, in DMOG treated chondrocytes. *P < 0.05, **P < 0.01, n = 4/group. Data were normalized to controls and presented as mean ± SEM.

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