Figure 3

GRP78, but not DENV E protein interacts with VDAC.

HEK293T/17 cells were mock infected or infected with DENV 2 or DENV 4, and cell lysates were prepared on 2 d.p.i. Co-immunoprecipitation assays were performed using an anti-VDAC antibody and western blot analysis was undertaken using (a) an anti-GRP78 antibody and (b) the same membrane was subsequently probed with a pan specific anti-dengue E antibody. (c) HEK293T/17 cells were mock transfected or transfected with eukaryotic expression plasmids harboring the sequences of flag peptide-tagged truncated DENV 4 E protein (Flag_D4ET) or EGFP protein as a transfection control, cells were collected at 2 days post transfection. Co-immunoprecipitation assays were performed using an anti-VDAC antibody and western blot analysis was performed using an antibody against the flag peptide.