Figure 4

Co-localization between GRP78 and VDAC.

(a) HEK293T/17 cells were mock infected or infected with DENV 2 or DENV 4 for 1 d.p.i. Then the cells were incubated with pan specific anti-dengue E protein antibody (blue), anti-GRP78 antibody (red) and anti-VDAC antibody (green) followed by appropriate secondary antibodies and DAPI (white). The cells were observed under Olympus Fluo View 1000 confocal microscope. Representative non-contrast adjusted merged and unmerged images are shown. (b) Representative non-contrast adjusted merged images of GRP78 and VDAC isolated from (a) are shown overlaid with phase-contrast image. (c) Isolated representative non-contrast adjusted merged images of VDAC and DENV E protein isolated from (a).