Figure 8

Role of VDAC in DENV infection.

(a) HEK293T/17 cells were mock transfected or transfected with si_hVDAC1 or si-GFP and subsequently infected with DENV 2 and DENV 4. Mock infected cells were run in parallel. On day 2 p.i. cell lysates were prepared and expression of DENV NS5, NS3, E and NS1 proteins together with VDAC and GAPDH were determined by western blot analysis. Experiments were undertaken independently in triplicate and protein band intensity from were quantitated using the imageJ image analysis program and analyzed by GraphPad Prism 5 progarm. The levels of (b) DENV 2 NS5, (c) DENV 2 and 4 NS3, (d) DENV 2 and 4 E protein, (e) DENV 2 NS1, and (f) VDAC were normalized to GAPDH. Error bars show S.E.M.