Table 1 Kinetics of IBT22-GFP-H6 protein depots remaining in tumor and soluble species released to plasma, and their antitumor effect in tumor tissue.

From: Bacterial mimetics of endocrine secretory granules as immobilized in vivo depots for functional protein drugs

Parameter

Time

0 hours

5 hours

3 days

7 days

IBs in tumor (μg)

200

184.5

155.3

87.9

IHC GFP H-score

N.d

140 ± 5

120 ± 12

89 ± 15

Cleaved-Caspase 3

N.d

3.2 ± 0.3

3.7 ± 0.6

3.5 ± 0.4

Apoptosis

N.d

3.7 ± 0.2

5.9 ± 1

8.0 ± 0.2

Mitosis

N.d

4.9 ± 0.3

3.9 ± 0.7

3.1 ± 0.1

Plasma (μg/ml)

0

2.9

16.4

0

  1. Samples were analyzed at 0 h, 5 h, 3 and 7 days after intratumoral injection at a 200 μg/mouse dose.
  2. IHC GFP Score: Immunohistochemical quantitation of proteins containing GFP in tumor tissue N.d.: not done.
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