Figure 6 | Scientific Reports

Figure 6

From: Novel Natural Product-like Caged Xanthones Bearing a Carbamate Moiety Exhibit Antitumor Potency and Anti-Angiogenesis Activity In vivo

Figure 6

(A) Morphological changes in cancer cells and nuclei. (B) 8n induced apoptosis in HepG2 cells. Cultured HepG2 cells were treated with the indicated concentrations of 8n for 48 h. The cells were then were stained with DAPI and observed by fluorescence microscopy. (C) 8n induces apoptosis in a dose-dependent manner. HepG2 cells were treated with different concentrations of 8n for 48 h, and Annexin V and propidium iodide staining were performed. (D) The percentages of early apoptotic and total apoptotic cells were quantified using flow cytometry. *p < 0.05; **p < 0.01. (E) The expression of apoptotic-related proteins in HepG2 cells treated with 8n. HepG2 cells were treated with various concentrations of 8n for 24 h and were then harvested and lysed. The expression levels of Caspase-3, Cleaved Caspase-3, Bax, Bcl-2, PARP and cleaved PARP were measured by Western blotting.

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