Figure 8

8n had anti-angiogenesis activity in vitro and in vivo.
(A) A wound-healing assay was used to evaluate the motility of HUVECs after treatment with 8n for 24 h. (B) Changes in cell motility were evaluated using a transwell assay of cell migration and invasion. (C) The effect of 8n on tube formation by HUVECs. HUVECs were incubated with 8n for 6 h and then transferred to Matrigel for 6 h. (D) Zebrafish embryos treated with blank control or 1, 10, or 50 μg/ml of 8n.