Figure 1 | Scientific Reports

Figure 1

From: Metformin sensitizes the response of oral squamous cell carcinoma to cisplatin treatment through inhibition of NF-κB/HIF-1α signal axis

Figure 1

Hypoxia potentiates chemoresistance to cisplatin in OSCC cells.

(A) HSC3, SCC3, and TCA8113 cells were treated with cisplatin under normoxic (20% O2) or hypoxic (1% O2) conditions for 48 h. The 50% inhibitory concentration (IC50) values of cells was analyzed by MTT assay. Absorbance was measured at 570 nm (A570). (B) Expression of HIF-1α mRNA in HSC3, SCC3, and TCA8113 cells transfected with or without HIF-1α siRNA under hypoxic conditions for 48 h. (C) Expression of HIF-1α protein in HSC3, SCC3, and TCA8113 cells transfected with or without HIF-1α siRNA under hypoxic conditions for 48 h. Full-length blots are presented in Supplementary Figure S1. (D) Expression of Bcl-2, GLUT-1, and HIF-1α in HSC3, SCC3, and TCA8113 cells transfected with or without HIF-1α siRNA under hypoxic conditions for 48 h. Full-length blots are presented in Supplementary Figure S2. (E) The IC50 values of cells transfected with or without HIF-1α siRNA were analysed by MTT assay under hypoxia. All experiments were repeated at least three times (*p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001).

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