Figure 1 | Scientific Reports

Figure 1

From: Evaluating the mobility potential of antibiotic resistance genes in environmental resistomes without metagenomics

Figure 1

Protocol for using IPCR to evaluate the horizontal gene transfer potential of ARGs in the environment.

After sample collection (1) and total DNA extraction (2), the DNA is digested with restriction enzymes and resulting fragments are self-ligated into circular DNA molecules (3). DNA flanking the ARG is amplified with IPCR using ARG targeting primers (4). The amplicons are sequenced using long read sequencing with PacBio SMRT cell technology and the ARG associated MGEs are identified (5).

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