Figure 2 | Scientific Reports

Figure 2

From: Evaluating the mobility potential of antibiotic resistance genes in environmental resistomes without metagenomics

Figure 2

Schematic representation of an IPCR amplicon resulting from digestion, self-circularization and amplification of DNA flanking a resistance gene.

Mobile genetic element is digested with restriction enzymes and self-ligated into a circular molecule (1) and amplified (2). The IPCR amplicon contains the up- and downstream regions surrounding the target ARG, which have MGE associated genes (mge1-5) and co-selected ARGs (co1 and co2). The digestion and ligation site separates the up-and downstream regions of the ARG in the IPCR amplicon.

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