Table 3 Sequences of primers used in IPCR.

From: Evaluating the mobility potential of antibiotic resistance genes in environmental resistomes without metagenomics

Primer

Sequence

Reference

sul1 inv FWa

AAGAACCGCACAATCTCGTC

42

sul1 inv RVa

GGCTTCCGCTATTGGTCTC

42

sul1 inv nest 1FWb

CGAAGAAGGAGTCCTCGGTG

This study

sul1 inv nest 2FWb

GACTCGCAGCATTTCGATCG

This study

sul1 inv nest 12RVb,c

TCGGAAACCCTCGCGAAATT

This study

sul1 inv nest 3FWb

CGACATCCACGACGTCTGAT

This study

sul1 inv nest 3RVb

GCCAGAGACCGAGGGTTAGA

This study

tetM inv FWa

CAGAATTGTTAGAGCCATATC

23

tetM inv RVa

GCAGAAATCAGTAGAATTGC

23

  1. asul1 inv FW and RV, and tetM inv FW and RV primers have been translated in to reverse complement from previously published primers
  2. bPrimers used in nested PCR have the word nest attached to the primer name
  3. csul1 inv nest 12RV was used with both sul1 inv nest 1FW and 2FW primers as the reverse primer.
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