Figure 2 | Scientific Reports

Figure 2

From: β-Arrestin 1’s Interaction with TC45 Attenuates Stat signaling by dephosphorylating Stat to inhibit antimicrobial peptide expression

Figure 2

Stat translocates into the nucleus to induce AMP expression against bacterial infection.

(A,B) Stat translocation in shrimp hemocytes challenged by V. anguillarum at 1 and 3 h was detected by immunocytochemistry using an anti-Stat antibody. (B) is the statistical analysis for the colocalization of Stat signal within the nucleus, as analyzed by WCIF ImageJ. (C,D) Stat phosphorylation in shrimp hemocytes challenged by V. anguillarum at 1 and 3 h, as detected by western blotting with an antibody recognizing phosphorylated Stat. (D) is the statistical analysis of C after digitization using Quantity One. (E) Western blotting was used to detect the RNAi efficiency of dsStat injection. (F) qRT-PCR detection of the expressions of CruI-1, CruI-5, ALF-A1, ALF-C1, ALF-C2 and ALF-D1 in Stat-silenced shrimp challenged by V. anguillarum at 6 h. (G,H) The bacteria clearance and survival rate of Stat-silenced shrimp were analyzed. dsGFP injection was used as the control. The experiments were repeats three times. The significant differences between two groups were analyzed using one-way ANOVA, followed by Tukey’s multiple comparison tests (*p < 0.05, **p < 0.01).

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