Table 1 Sequences of the primers used in this study.
Primer | Sequence (5′–3′) |
|---|---|
Tissue distribution and expression pattern analysis | |
βarr1-RT-F | TTTCACGCTGACGCCACT |
βarr1-RT-R | AGCAACCAGATCACCAACTAG |
βarr2-RT-F | TGGCTCTATTCCTCTGCG |
βarr2-RT-R | TGGCTCTATTCCTCTGCG |
TC45-RT-F | CTCCCTTACCCGCTCTATGA |
TC45-RT-R | TTTCTCTTGCCAATGTTCGT |
Stat-RT-F | GGTCCCAGTTCTGTAAGG |
Stat-RT-R | TAGGCACATTCGGATAAA |
β-actin F | CAGCCTTCCTTCCTGGGTATGG |
β-actin R | GAGGGAGCGAGGGCAGTGATT |
ALF-A1-RT-F | CTGGTCGGTTTCCTGGTGGC |
ALF-A1-RT-R | CCAACCTGGGCACCACATACTG |
ALF-C1-RT-F | CGCTTCAAGGGTCGGATGTG |
ALF-C1-RT-R | CGAGCCTCTTCCTCCGTGATG |
ALF-C2-RT-F | TCCTGGTGGTGGCAGTGGCT |
ALF-C2-RT-R | TGCGGGTCTCGGCTTCTCCT |
ALF-D1-RT-F | GCTTTTTATTTTGGGGGTCACGCTGT |
ALF-D1-RT-R | CTTTGGCGTGGAACAAGGTAGAGGAT |
CruI-1-RT-F | TGCTCAGAACTCCCTCCACC |
CruI-1-RT-R | TTGAATCAGCCCATCGTCG |
CruΙ-5-RT-F | TGCGAAACAGACAGGGATTGC |
CruΙ-5-RT-R | CCGAAGACCAGATGACCGAAA |
Recombinant expression | |
βarr1-ExF | TACTCAGGATCCATAATGGAGGACAACAG |
βarr1-ExR | TACTCACTCGAGTTCCTACGCCTCTGT |
βarr1-C-ExF | TACTCAGGATCCATAATGGAGGACAACAGC |
βarr1-C-ExR | TACTCACTCGAGGCATACATGACCTTCCTG |
βarr1-N-ExF | TACTCAGGATCCCAAGGAGAACAGCCAAGTGTG |
βarr1-N-ExR | TACTCACTCGAGTTCCTACGCCTCTGTTTCTCC |
TC45-ExF | TACTCAGAATTCGTCAGTATGCCTACAAATCTA |
TC45-ExR | TACTCACTCGAGCTGGACACGAGGACGCTT |
Stat(1aa)-ExF | TACTCAGAATTCATGTCGTTGTGGAACAGAGC |
Stat(800aa)-ExR | TACTCACTCGAGTTACAAATGCCGGTGAACATA |
Stat(507aa)-ExR | TACTCACTCGAGAGCATTATCCCATGA |
Stat(507aa)-ExF | TACTCAGGATCCACAGTGTCATGGGAT |
Stat(598aa)-ExR | TACTCACTCGAGCAGCATCTCTTCAGC |
Stat(598aa)-ExF | TACTCACTCGAGAAGTACTACACGCCA |
RNA interference | |
βarr1-Fi | GCGTAATACGACTCACTATAGGCTGATTCAAGTCAAAAGGAG |
βarr1-Ri | GCGTAATACGACTCACTATAGGGAATCTTCCTTTCTGTTCAAC |
βarr2-Fi | GCGTAATACGACTCACTATAGGTCCTCTGCGTGAACATTTTCC |
βarr2-Ri | GCGTAATACGACTCACTATAGGCTTCTCTTATATCTCCTCGA |
TC45-Fi | GCGTAATACGACTCACTATAGGATGGTGCACATAAGCTA |
TC45-Ri | GCGTAATACGACTCACTATAGGCACAATTCATGTAGGA |
Stat-Fi | GCGTAATACGACTCACTATAGGGACTTTCCTGCTCCGTTTC |
Stat-Ri | GCGTAATACGACTCACTATAGGGCGTTGGCACTGTTGAGAC |
GFP-Fi | GCGTAATACGACTCACTATAGGTGGTCCCAATTCTCGTGGAAC |
GFP-Ri | GCGTAATACGACTCACTATAGGCTTGAAGTTGACCTTGATGCC |
