Table 1 Characteristics of the oligonucleotides used in simulations.

From: Synthetic oligonucleotide antigens modified with locked nucleic acids detect disease specific antibodies

Sequence, 5′-3′

nr./position (LNAs)

Length (nt)

Base content

Sequences used in molecular modelling

 SEQ1: TCC + TCT CTT TCT + CTT TCT + CTT

3/4, 13, 19

21

62% T, 38% C

 SEQ2: ATT + TAT TTT + TAT ATT TAT + ATT

3/4, 10, 19

21

71% T, 29% A

 SEQ3: TGA ACT + CTA TGT + CTG TAT + CAT

3/7, 13, 19

21

43% T, 24% A 19% C, 14% G

 SEQ4: TCC + TCT CTT + TCT + CTT TCT + CTT

4/4, 10, 14, 19

21

62% T, 38% C

 SEQ5: TCC TCT CTT TCT CTT TCT CTT

0/−

21

62% T, 38% C

  1. The five oligonucleotides have different sequences and additional LNA in SEQ1-SEQ4*.
  2. *Pluses indicate LNA modifications. Bold indicate docking bases.
Back to article page