Figure 3
Differential pathway and gene expression activity across monkey and human ECs and IPSCs.
(A) Heat map of differentially activated pathways. Columns represent gene ontology terms for biological processes and pathways, and rows are monkey and human ECs and IPSCs. Pathway enrichment scores were calculated on the whole transcriptome expression profiles for each sample using gene set variation analysis; blue indicates lower and red indicates higher pathway activity throughout the sample population. Hierarchical clustering of pathways and samples is based on complete linkage and Pearson correlation distance. (B) Heat map of genes annotated by the biological process of endothelium development (GO:0003158). Columns represent genes, and rows are monkey and human ECs and IPSCs. Column Z-score transformation was performed on log2 values for each gene, with blue denoting lower and red denoting higher expression levels according to the average expression level. Hierarchical clustering regarding the biological process shows clustering of monkey IPSC-derived ECs with high similarity to human IPSC-derived ECs and primary human ECs. (C) Heat map of marker gene panels for pluripotency and endothelial cells of the same samples as in (B). Columns represent genes and rows are samples. Column Z-score transformation was performed on log2 values for each gene. Hierarchical clustering of genes and samples is based on complete linkage and Pearson correlation distance.
