Figure 4
Monkey and human IPSC-derived endothelial cells as an in vitro model for inter-species comparisons.
(A) Left: Scatterplot of barrier function genes involved in cell-cell-junction formation. Gene expression values for cIPSC-ECs compared to hIPSC-ECs are shown as the mean log2-transformed fragments per million mapped fragments (FPM + 0.1) of 3 replicates. Dashed lines indicate a fourfold cut-off in expression level difference. The Pearson correlation coefficient (r) between samples is shown at the upper left corner. Right: Impedance-based measurements of the EC monolayer reveal no species-specific differences between hIPSC-ECs and cIPSC-ECs. One of the 2 independent experiments performed is depicted (n = 4 technical replicates). Columns show the mean of 4 technical replicates +/− STD. (B) Left: Scatterplot of genes annotated by the biological process for positive regulation of cytokine secretion (GO:0050715). Gene expression values for cIPSC-ECs compared to hIPSC-ECs are shown as the mean log2-transformed fragments per million mapped fragments (FPM + 0.1) of 3 replicates. Dashed lines indicate the four-fold cut-off in expression level difference. The Pearson correlation coefficient (r) between samples is shown in the upper left corner. Right: Functional validation of gene expression analysis by analyzing cytokine release upon stimulation with IL1β. Twenty-four hours after treatment with 10 ng/ml IL1β, the release of proinflammatory cytokines was measured using multiplex ELISA. Columns show mean +/− STD of six biological replicates.
