Figure 3

Seminal fluid protein abundance variation associated with sperm velocity in old males.
(A) 3-dimension visualization of Principle Component Analysis (PCA3), using the inclusive protein dataset as variables (Old Fast, blue; Old Slow; black; Young Fast; green; Young Slow, yellow). Dimension 1 captures variance associated with old male sperm velocity, including negative weightings for Old Slow and positive weightings for Old Fast. (B) Histogram displaying the distribution of first dimension weightings for proteins exhibiting significant abundance differences between the Slow and Fast sample of the replicated old male (#308)(abundance increase in fast sample, blue; abundance increase in slow sample, black). (C) Histogram displaying the excess or deficit of blood plasma proteins (red) and exosome markers (black) across first dimension weight ranges.