Figure 4
From: von Willebrand factor contributes to poor outcome in a mouse model of intracerebral haemorrhage
VWF increased inflammatory cell recruitment, ICAM-1 and MMP-9 levels after ICH.
(A,C) Representative images of microglia and neutrophils immunostaining in the perihematomal area 24 hours after ICH in mice treated with either intraventricular (ICV) or intravenous (IV) injection of VWF. Nuclei were visualized with DAPI. Bar = 15 μm. (B,D) Quantitative analysis of activated microglia and neutrophils in the perihematomal area. Values are means ± standard errors of the means (n = 5). *P < 0.05. Microglia were considered activated when the cell body exhibited stout morphology with retracted processes. (E) Representative immunoblots and quantification of ICAM-1 in the brain extracts from mice treated with vehicle or VWF 24 hours after ICH. Values are means ± standard errors of the means (n = 5). (F) Representative MMP-9 gelatin zymography and quantitative analysis of pro-MMP-9 and active-MMP-9 in the brain extracts from mice treated with vehicle or VWF 24 hours after ICH. Sd, Human MMP-9. Values are means ± standard errors of the means (n = 5). *P < 0.05.
