Figure 5
From: von Willebrand factor contributes to poor outcome in a mouse model of intracerebral haemorrhage
Antibodies against VWF improved neurological functions and reduced brain edema after ICH.
(A) Distribution of the injected anti-VWF antibody in the brain was labeled with an anti-rabbit secondary antibody. (B) Representative photographs of cerebral coronal sections show Evans blue dye extravasation (blue staining) 24 hours after ICH in mice treated with control rabbit IgG or rabbit anti-VWF antibody. (C) Quantification of Evans blue dye extravasation by spectrophotometric assay. Values are means ± standard errors of the means (n = 8). *P < 0.05. (D) Brain edema measured 72 hours after ICH in mice treated with control rabbit IgG or rabbit anti-VWF antibody. Ipsi, ipsilateral hemisphere; Contral, contralateral hemisphere; Cereb, cerebellum. Values are means ± standard errors of the means (n = 8). *P < 0.05. (E) Hemorrhagic volume measured 24 hours after ICH by spectrophotometric hemoglobin assay in mice treated with control rabbit IgG or rabbit anti-VWF antibody. Values are means ± standard errors of the means (n = 5). NS, not significant. (F) Tail bleeding time measured 24 hours after ICH in mice treated with control rabbit IgG or rabbit anti-VWF antibody. *P < 0.05. (G) Neurological score in mice treated with control rabbit IgG or rabbit anti-VWF antibody 72 hours after ICH. Values are means ± standard errors of the means (n = 8). *P < 0.05.
