Figure 1

Characterization of phosphopeptides and phosphosites at the Log₂ (5/6Nx + NS)/(Sham + NS) or Log₂(5/6Nx + HS)/(5/6Nx + NS) ratio in (5/6Nx + NS)/(Sham + NS) and (5/6Nx + HS)/(5/6Nx + NS) comparison groups.

(A) Distribution of singly, doubly, triply and quadruply phosphorylated peptides; (B) Distribution of the Ser, Thr, Tyr phosphosites in singly phosphorylated peptides. Phosphopeptides were categorized into class I phosphosites by calculating the probabilities for phosphorylation at each site based on posttranslational modification scores. Here, only class I phosphosites (high probability) were used to analyze the distribution. 1P, 2P, 3P and 4P represent singly, doubly, triply and quadruply phosphorylated peptides, respectively. Sham + NS, sham operation + normal salt; 5/6Nx + NS, 5/6Nx + normal salt; 5/6Nx + HS, 5/6Nx + high salt.