Figure 3 | Scientific Reports

Figure 3

From: Cattle encephalon glycoside and ignotin reduced white matter injury and prevented post-hemorrhagic hydrocephalus in a rat model of intracerebral hemorrhage

Figure 3

Immunofluorescence staining of myelin basic protein (MBP) and microtubule associated protein 2 (MAP-2) to assess axon and myelin sheath damage 14 days post-ICH induction.

(A) ICH and ICH + CEGI brain sections were counterstained with DAPI and examined for remyelination using the axonal marker MAP-2 (green) and the myelin sheath marker MBP (red). The axonal and myelin damage in the subcortical white matter was alleviated in the ICH + CEGI 4 ml/kg group at day 14 post-ICH induction. (B) Location of observed field. (C) Temporal changes in density of axons (MAP-2+) in ICH and ICH + CEGI 4 ml/kg groups at 7 and 14 days post-ICH induction. (D) Temporal changes in density of myelinated cluster in ICH and ICH + CEGI 4 ml/kg groups at 7 and 14 days post-ICH induction. *p < 0.05 for ICH vs. sham; #p < 0.05 for ICH + CEGI 4 ml/kg vs. ICH. n = 6 per group at each time point.

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