Table 1 Properties of green fluorescent proteins tested.

From: Expression of varied GFPs in Saccharomyces cerevisiae: codon optimization yields stronger than expected expression and fluorescence intensity

Protein

λex (nm)

λem (nm)

EC (ε) (M−1cm−1)

QY

RB (% of EGFP)

Association state

Reference or source

EGFP

488

507

56,000

0.60

100

Monomer

18

AcGFP1

475

505

32,500

0.82

79

Monomer

Clontech

TagGFP2

483

506

56,500

0.60

101

Monomer

Evrogen

mUkG1

483

499

60,000

0.72

129

Monomer

26

ZsGreen

493

505

43,000

0.91

116

Tetramer

27

mWasabi

493

509

70,000

0.80

167

Monomer

29

mNeonGreen

506

517

116,000

0.80

276

Monomer

24

  1. Along with the common name for each GFP, the peak excitation (λex) and emission (λem) wavelengths, molar extinction coefficient (EC), quantum yield (QY), relative brightness (RB), association state and reference or source are listed. Brightness values were derived from the product of EC and QY, divided by the value for EGFP.
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