Table 2 List of plasmids used in this study.

Expression vector containing PGK1 promoter, CEN/ARS single-copy origin and URA3 marker

33

EGFP expression, in pGK416

33

AcGFP1 expression, in pGK416

This study

TagGFP2 expression, in pGK416

This study

mUkG1 expression, in pGK416

This study

ZsGreen expression, in pGK416

34

yEGFP (codon-optimized EGFP) expression, in pGK416

This study

yAcGFP1 (codon-optimized AcGFP1) expression, in pGK416

This study

yTagGFP2 (codon-optimized TagGFP2) expression, in pGK416

This study

ymUkG1 (codon-optimized mUkG1) expression, in pGK416

This study

yZsGreen (codon-optimized ZsGreen) expression, in pGK416

This study

ymWasabi (codon-optimized mWasabi) expression, in pGK416

This study

ymNeonGreen (codon-optimized mNeonGreen) expression, in pGK416

This study

EGFP-FLAG expression, in pGK416

This study

AcGFP1-FLAG expression, in pGK416

This study

TagGFP2-FLAG expression, in pGK416

This study

mUkG1-FLAG expression, in pGK416

This study

ZsGreen-FLAG expression, in pGK416

This study

yEGFP (codon-optimized EGFP) -FLAG expression, in pGK416

This study

yAcGFP1 (codon-optimized AcGFP1) -FLAG expression, in pGK416

This study

yTagGFP2 (codon-optimized TagGFP2) -FLAG expression, in pGK416

This study

ymUkG1 (codon-optimized mUkG1) -FLAG expression, in pGK416

This study

yZsGreen (codon-optimized ZsGreen)-FLAG expression, in pGK416

This study

ymWasabi (codon-optimized mWasabi)-FLAG expression, in pGK416

This study

ymNeonGreen (codon-optimized mNeonGreen)-FLAG expression, in pGK416

This study

Cloning vector

Agilent Technologies

P_FIG1 (300 bp)-URA3-ZsGreen-T_FIG1 (200 bp) in pBlueScript II KS(+)

34

T_FIG1 (200 bp) in pBlueScript II KS(+)

This study

C-terminus of FIG1 (50 bp)-AcGFP1-URA3-T_FIG1 (200 bp) in pBlueScript II KS(+)

This study

C-terminus of FIG1 (50 bp)-TagGFP2-URA3-T_FIG1 (200 bp) in pBlueScript II KS(+)

This study

C-terminus of FIG1 (50 bp)-mUkG1-URA3-T_FIG1 (200 bp) in pBlueScript II KS(+)

This study

C-terminus of FIG1 (50 bp)-ZsGreen-URA3-T_FIG1 (200 bp) in pBlueScript II KS(+)

This study

C-terminus of FIG1 (50 bp)-yEGFP-URA3-T_FIG1 (200 bp) in pBlueScript II KS(+)

This study

C-terminus of FIG1 (50 bp)-yAcGFP1-URA3-T_FIG1 (200 bp) in pBlueScript II KS(+)

This study

C-terminus of FIG1 (50 bp)-yTagGFP2-URA3-T_FIG1 (200 bp) in pBlueScript II KS(+)

This study

C-terminus of FIG1 (50 bp)-ymUkG1-URA3-T_FIG1 (200 bp) in pBlueScript II KS(+)

This study

C-terminus of FIG1 (50 bp)-yZsGreen-URA3-T_FIG1 (200 bp) in pBlueScript II KS(+)

This study

Expression vector containing PGK1 promoter, 2 μ origin and URA3 marker

33

URA3-P_STE18-kanMX4-T_STE18 in pGK426

42

URA3-P_STE18-Gγ_cyto-T_PGK1-T_HIS3 in pGK426

45

URA3-P_STE18-Gγ_cyto-Fc-T_PGK1 in pUSTE18p-Gγcyto-HIS3t

42

Expression vector containing PGK1 promoter, CEN/ARS single-copy origin and HIS3 marker

33

Z_WT and C-terminus of Ste18 (9 a.a.) fusion expression, in pGK413

40

Z_K35A and C-terminus of Ste18 (9 a.a.) fusion expression, in pGK413

40

Z_I31A and C-terminus of Ste18 (9 a.a.) fusion expression, in pGK413

40

Z₉₅₅ and C-terminus of Ste18 (9 a.a.) fusion expression, in pGK413

40

URA3-P_STE18-kanMX4-T_STE18 in pGK426

42

URA3-P_STE18-P_PGK1-Gpa1N (9 a.a.)-Fc-T_PGK1-kanMX4-T_STE18 in pGK426-GPTK

45

URA3-P_STE18-P_PGK1-Fc-Ste18C (9 a.a.)-T_PGK1-kanMX4-T_STE18 in pGK426-GPTK

45

URA3-P_STE18-Gγ_cyto-T_PGK1-T_HIS3 in pGK426

45

URA3-P_STE18-Gγ_cyto-Z_WT-T_PGK1 in pUSTE18p-Gγcyto-HIS3t

45

URA3-P_STE18-Gγ_cyto-Z_K35A-T_PGK1 in pUSTE18p-Gγcyto-HIS3t

45

URA3-P_STE18-Gγ_cyto-Z_I31A-T_PGK1 in pUSTE18p-Gγcyto-HIS3t

45

URA3-P_STE18-Gγ_cyto-Z₉₅₅-T_PGK1 in pUSTE18p-Gγcyto-HIS3t

45