Table 3 Yeast strains used in this study.
Strain | Relevant feature | Reference |
|---|---|---|
BY4741 | MATa his3Δ1 ura3Δ0 leu2Δ0 met15Δ0 | |
BY4742 | MATα his3Δ1 ura3Δ0 leu2Δ0 lys2Δ0 | |
MC-F1 | BY4741 fig1::FIG1-EGFP | |
BYFAG1 | BY4741 fig1::FIG1-AcGFP1 | This study |
BYFTG1 | BY4741 fig1::FIG1-TagGFP2 | This study |
BYFUG1 | BY4741 fig1::FIG1-mUkG1 | This study |
BYFZG1 | BY4741 fig1::FIG1-ZsGreen | This study |
BYFEG2 | BY4741 fig1::FIG1-yEGFP (codon optimized EGFP) | This study |
BYFAG2 | BY4741 fig1::FIG1-yAcGFP1 (codon optimized AcGFP1) | This study |
BYFTG2 | BY4741 fig1::FIG1-yTagGFP2 (codon optimized TagGFP2) | This study |
BYFUG2 | BY4741 fig1::FIG1-ymUkG1 (codon optimized mUkG1) | This study |
BYFZG2 | BY4741 fig1::FIG1-yZsGreen (codon optimized ZsGreen) | This study |
BFG2118 | MC-F1 ste18Δ::kanMX4 his3Δ::URA3-PSTE18-Gγcyto-Fc | |
UGW2 | BYFUG2 ste18Δ::kanMX4 | This study |
UGFG2 | BYFUG2 ste18Δ::kanMX4 his3Δ::URA3-PSTE18-Gγcyto-Fc | This study |
MC-FC | MC-F1 ste18Δ::kanMX4-PPGK1-Fc-Ste18C | |
MC-FN | MC-F1 ste18Δ::kanMX4-PPGK1-Gpa1N-Fc | |
FC-GW | MC-F1 ste18Δ::kanMX4-PPGK1-Fc-Ste18C his3Δ::URA3-PSTE18-Gγcyto-ZWT | |
FC-GK | MC-F1 ste18Δ::kanMX4-PPGK1-Fc-Ste18C his3Δ::URA3-PSTE18-Gγcyto-ZK35A | |
FC-GI | MC-F1 ste18Δ::kanMX4-PPGK1-Fc-Ste18C his3Δ::URA3-PSTE18-Gγcyto-ZI31A | |
FC-G9 | MC-F1 ste18Δ::kanMX4-PPGK1-Fc-Ste18C his3Δ::URA3-PSTE18-Gγcyto-Z955 | |
FC-G0 | MC-F1 ste18Δ::kanMX4-PPGK1-Fc-Ste18C his3Δ::URA3 | |
FN-GW | MC-F1 ste18Δ::kanMX4-PPGK1-Gpa1N-Fc his3Δ::URA3-PSTE18-Gγcyto-ZWT | |
FN-GK | MC-F1 ste18Δ::kanMX4-PPGK1-Gpa1N-Fc his3Δ::URA3-PSTE18-Gγcyto-ZK35A | |
FN-GI | MC-F1 ste18Δ::kanMX4-PPGK1-Gpa1N-Fc his3Δ::URA3-PSTE18-Gγcyto-ZI31A | |
FN-G9 | MC-F1 ste18Δ::kanMX4-PPGK1-Gpa1N-Fc his3Δ::URA3-PSTE18-Gγcyto-Z955 | |
FN-G0 | MC-F1 ste18Δ::kanMX4-PPGK1-Gpa1N-Fc his3Δ::URA3 | |
BYFUG2-FC | BYFUG2 ste18Δ::kanMX4-PPGK1-Fc-Ste18C | This study |
BYFUG2-FN | BYFUG2 ste18Δ::kanMX4-PPGK1-Gpa1N-Fc | This study |
UG2-FCGW | BYFUG2 ste18Δ::kanMX4-PPGK1-Fc-Ste18C his3Δ::URA3-PSTE18-Gγcyto-ZWT | This study |
UG2-FCGK | BYFUG2 ste18Δ::kanMX4-PPGK1-Fc-Ste18C his3Δ::URA3-PSTE18-Gγcyto-ZK35A | This study |
UG2-FCGI | BYFUG2 ste18Δ::kanMX4-PPGK1-Fc-Ste18C his3Δ::URA3-PSTE18-Gγcyto-ZI31A | This study |
UG2-FCG9 | BYFUG2 ste18Δ::kanMX4-PPGK1-Fc-Ste18C his3Δ::URA3-PSTE18-Gγcyto-Z955 | This study |
UG2-FCG0 | BYFUG2 ste18Δ::kanMX4-PPGK1-Fc-Ste18C his3Δ::URA3 | This study |
UG2-FNGW | BYFUG2 ste18Δ::kanMX4-PPGK1-Gpa1N-Fc his3Δ::URA3-PSTE18-Gγcyto-ZWT | This study |
UG2-FNGK | BYFUG2 ste18Δ::kanMX4-PPGK1-Gpa1N-Fc his3Δ::URA3-PSTE18-Gγcyto-ZK35A | This study |
UG2-FNGI | BYFUG2 ste18Δ::kanMX4-PPGK1-Gpa1N-Fc his3Δ::URA3-PSTE18-Gγcyto-ZI31A | This study |
UG2-FNG9 | BYFUG2 ste18Δ::kanMX4-PPGK1-Gpa1N-Fc his3Δ::URA3-PSTE18-Gγcyto-Z955 | This study |
UG2-FNG0 | BYFUG2 ste18Δ::kanMX4-PPGK1-Gpa1N-Fc his3Δ::URA3 | This study |
