Figure 5 | Scientific Reports

Figure 5

From: Bioinspired seeding of biomaterials using three dimensional microtissues induces chondrogenic stem cell differentiation and cartilage formation under growth factor free conditions

Figure 5

Microaggregation results in an improved chondrogenic gene expression fingerprint after three weeks in culture.

hPDCs were cultured as single cells or microaggregates of 100 cells for a period of 21 days and analyzed on their respective gene expression of SOX9, ACAN, COL2A1, COL1A1 and COLX. Moreover, their (B) collagen and (C) glycosaminoglycan (GAG) were measured on various time points and normalized to their DNA content, which demonstrated significant elevations in collagen and GAG production. Data is represented as mean ± SD. N = 4. *P < 0.05; **P < 0.01.

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