Figure 3

Delineation of tumor margin with TRI.

(a) TRI image of a WHO grade II glioma specimen (case 6). The red and green regions denote tumor regions exceeding TH1 and TH2. The graph below TRI image shows the display for terahertz parameter value along the dashed line A and B. The green region on right of specimen is not tumor because the region is located in gray matter. (b) White light image of the specimen. (c) Tumor margins (yellow dashed line) determined by H&E stained image. The result agreed well to the tumor margin detected by TRI image. (d) Magnified H&E image (x12.5) of the black dashed box region in (c). (e) A further magnified image (x40) of black dashed box region in d, in which tumor cells could not be identified. (f) However, immunohistochemistry showed the presence of IDH1 mutated cells (brown) on the same region of (e), which have been known to be indicated glioma cells. A further magnified immunohistochemistry image (x400) of some region in (e). (g) Based on the IDH1 mutation stained image, the tumor margins (blue dots) should be re-delineated beyond the margins determined by H&E stained image.