Figure 2
MALAT1 combined with HULC accelerlates liver cancer stem cell growth in vitro.
(a) The RT-PCR analysis of MALAT1 and HULC in stable liver cancer stem cell lines transfected with pCMV6-A-GFP, pCMV6-A-GFP-HULC, pCMV6-A-GFP-MALAT1, pCMV6-A-GFP-HULC plus pCMV6-A-GFP-MALAT1 β-actin as internal control. (b) Cells growth assay using CCK8. Each value was presented as mean ± standard error of the mean (SEM). (c) S phase cells assay using BrdU. Each value was presented as mean ± standard error of the mean (SEM). (d) Cells soft agar colony formation assay. Each value was presented as mean ± standard error of the mean (SEM). (e) Cells Transwell assay. Each value was presented as mean ± standard error of the mean (SEM)). (f) Wound healing assay. Each value was presented as mean ± standard error of the mean (SEM).
