Figure 6
MALAT1 combined with HULC enhanced telomerase activity.
(a) TERC promoter methylation analysis by MspI plus BamHI digestion in liver cancer stem cell lines transfected with pCMV6-A-GFP, pCMV6-A-GFP-HULC, pCMV6-A-GFP-MALAT1, pCMV6-A-GFP-HULC plus pCMV6-A-GFP-MALAT1 (b) (upper) Co-Immunoprecipitation (CO-IP) with anti-TERT followed by Western blotting with anti-TRF2. IgG IP as negative control. Western blotting with anti-TERT as INPUT. (lower) RNA Immunoprecipitation (RIP) with anti-TERT followed by RT-PCR with TERC mRNA primers. IgG RIP as negative control. TERC mRNA as INPUT. (c) RNA Immunoprecipitation (RIP) with anti-TERT followed by RT-PCR with TERC mRNA primers in stable liver cancer stem cell lines transfected with pGFP-V-RS, pGFP-V-RS-HULC, pGFP-V-RS–MALAT1, pGFP-V-RS-HULC plus pGFP-V-RS-MALAT1. IgG RIP as negative control. TERC mRNA as INPUT. (d) The telomerase activity was measured by using Quantitative Telomerase Detection. Each value was presented as mean ± standard error of the mean (SEM).
