Figure 7
MALAT1 combined with HULC increases Microsatallite Instability (MSI) and cell cycle related protein interplay.
(a) Microsatallite Instability (MSI) analysis through Dot blot (Slot blot) using various Biotin labling MSI probes (Biotin-MSIs) primers in liver cancer stem cell lines transfected with pCMV6-A-GFP, pCMV6-A-GFP-HULC, pCMV6-A-GFP-MALAT1, pCMV6-A-GFP-HULC plus pCMV6-A-GFP-MALAT1. (b) (upper) Co-Immunoprecipitation (IP) with anti-RFC1 followed by Western blotting with anti-PCNA. IgG IP as negative control. Western blotting with anti-PCNA as INPUT. (lower) Co-Immunoprecipitation (IP) with anti-CDK2 followed by Western blotting with anti-CyclinE. IgG IP as negative control. Western blotting with anti-CyclinE as INPUT.
