Figure 8
The rescued experiment of carcinogenesis effect of the MALAT1 combined with HULC.
TRF2 knockdown abrogated the oncogenic function of the MALAT1 combined with HULC. (a) The RT-PCR analysis of MALAT1, HULC (upper) and the western blotting analysis with anti-TRF2(lower) in stable liver cancer stem cell lines transfected with pCMV6-A-GFP, pCMV6-A-GFP-HULC plus pCMV6-A-GFP-MALAT1, pCMV6-A-GFP-HULC plus pCMV6-A-GFP-MALAT1 plus pcDNA3.1-TRF2, pGFP-V-RS-GFP-HULC plus pGFP-V-RS–MALAT1 plus pcDNA-CREPT. β -actin as internal control. (b) Cells growth assay using CCK8. Each value was presented as mean ± standard error of the mean (SEM). (c) Cells soft agar colony formation assay. Each value was presented as mean ± standard error of the mean (SEM). (d) In vivo test in stable liver cancer stem cell lines transfected with pCMV6-A-GFP, pCMV6-A-GFP-HULC plus pCMV6-A-GFP-MALAT1, pCMV6-A-GFP-HULC plus pCMV6-A-GFP-MALAT1 plus pcDNA3.1-TRF2, pGFP-V-RS-GFP-HULC plus pGFP-V-RS–MALAT1 plus pcDNA-CREPT. The mice were stratified and the tumors were recovered. The photography of xerograft tumor in the four groups (indicated in left). (e) The wet weight of each tumor was determined for each mouse. Each value was presented as mean ± standard error of the mean (SEM). (f) The Xenograft appearance time. Each value was presented as mean ± standard error of the mean (SEM). (g) PCNA expression in the Xenografts. Each value was presented as mean ± standard error of the mean (SEM).
