Figure 1 | Scientific Reports

Figure 1

From: Isolated exopolysaccharides from Lactobacillus rhamnosus GG alleviated adipogenesis mediated by TLR2 in mice

Figure 1

The effects of cell extract from LGG on the adipogenesis (A,B) and inflammation (C,D) in 3T3-L1 adipocytes. 3T3-L1 cells from initiating differentiation (Day 0) to terminate mature (Day 6) as indicated in methods were treated with the supernatant of cell extracts (40 μg/ml) from LGG or water as the control. (A) Effects of four fractions of LGG extract (<10 KD, 10–30 KD, 30–50 KD and >50 KD) on TAG accumulation in 3T3-L1 adipocyte. (B) Effects of LGG extracts after boiling or autoclaving on TAG accumulation in 3T3-L1 adipocyte. The expression of M1 proinflammatory genes (C) and M2 anti-inflammatory genes (D) in 3T3-LI cells by q-PCR. Data were expressed as mean ± SEM of three independent experiments (n = 3). Significance was established using a two-tailed Student’s t-test. Differences were considered significant at P < 0.05 (*) and P < 0.01 (**).

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