Figure 7
LGG EPS inhibited adipogenesis in 3T3-L1 cells via Toll-like receptor-2.
(A,B) The effects of LGG EPS on the expression of TLRs in mature 3T3-L1 adipocytes (A) and gonadal white adipose tissue of mice (B). (C) Knockdown of TLR2 expression in 3T3-L1 cells with transfection of siRNA reversed lipid accumulation as assessed by Oil Red O staining. (D) Quantification of lipid accumulation of differentiated cells by TAG assay. (E) Knockdown of TLR2 reverses the expression of adipocyte markers measured by real-time PCR. Data were expressed as mean ± SEM of three independent experiments (n = 3). Significance was established using a two-tailed Student’s t-test. Differences were considered significant at P < 0.05 (*) and P < 0.01 (**).
