Figure 1
From: Targeting inflammasome/IL-1 pathways for cancer immunotherapy
Inflammasome deficient mice exhibit reduced tumor growth and metastasis.
(A) WT C57BL/6 mice and caspase-1 KO mice (n = 6) were injected orthotopically with 2.5 × 105 EO771 mammary tumor cells. Tumor growth was monitored. (B) Four weeks after tumor inoculation, the lungs of tumor-bearing mice were harvested, and the visible tumor nodules were counted. Symbols in scatter plots represent the number of tumor nodules in the lung from individual mouse, and results are expressed as mean ± SD. Data are representative of three independent experiments with similar results. (C) Representative H&E staining of lung sections for spontaneous lung metastasis four weeks after tumor inoculation. Scale bars: 200 μm. Arrows mark metastases in lungs. (D) WT and caspase-1 KO mice (n = 5) were injected intravenously with EO771 cells. Mice were sacrificed on day 21 post-inoculation. Lungs were harvested, and metastasis nodules were counted. Symbols in scatter plots represent the number of tumor nodules in the lung, and results are expressed as mean ± SD. Lower panel, gross morphology of lungs of WT or mutant mice injected i.v. with EO771 cells. Arrows mark visible surface metastases in lungs. *P < 0.05, **P < 0.01 (Student’s t test). (E) Survival curves of WT and caspase-1 KO mice (n = 6) injected intravenously with 2 × 105 EO771 mammary tumor cells. *P < 0.05, statistical comparisons were performed by two-way ANOVA analysis. Data are representative of three independent experiments with similar results.
