Figure 3
(+)-Naloxone prevents E. coli-induced PTD and fetal loss.
Pregnant B6 mice were administered intrauterine heat-killed E. coli or PBS on gd 16.5, followed by (+)-naloxone or PBS i.p. on gd 16.5, 17.0, 17.5 and 18.0, then observed for PTD and allowed to progress to birth. (A) The percentage of pregnant dams exhibiting PTD, analyzed by χ2 test. (B) The gestation length, (C) number of viable pups born per dam, (D) pup weight at 24 h, (E) pup survival to 3 weeks and (F) weight of surviving pups at 3 weeks. (B,C,E) Data are mean ± SEM analyzed by two-way ANOVA, and (D,F) data are estimated marginal mean ± SEM, analysed by Mixed Model Linear Repeated Measures ANOVA and post-hoc LSD t-test, with mother as the subject. a,b,cDifferent letters indicate differences between groups, p < 0.05. *Significant effect of E. coli, independently of (+)-naloxone, p < 0.001. Number of pregnant dams per group is shown in parentheses.
