Figure 4
(+)-Naloxone suppresses E. coli–induced pro-inflammatory cytokine expression in placenta and fetal membranes.
Pregnant B6 mice were administered intrauterine heat-killed E. coli or PBS on gd 16.5, followed by (+)-naloxone or PBS i.p., and 4 hours later, placenta and fetal membranes were recovered from the two adjacent implantation sites. Relative expression of Il1a (A,F), Il1b (B,G), Il6 (C,H), Tnf (D,I) and Il10 (E,J) mRNAs were determined in placenta (A–E) and fetal membranes (F–J) by qPCR and normalised to Actb. Data are mean ± SEM relative gene expression of n = 12 tissues from n = 6 dams/group and were analysed by one-way ANOVA and post-hoc Sidak t-test. a,b,cDifferent letters indicate differences between groups, p < 0.05.
