Figure 4
JB increases intracellular ROS production and mitochondrial damage in B16F10 cells.
The cells were treated with the indicated concentrations of JB, and then mitochondrial membrane potential and intracellular ROS level were measured using Flow Cytometry. (a) The representative images of mitochondrial membrane potential determined by Flow Cytometry. The cells with green-positive and red-negative fluorescence were counted as depolarized cells. (b) The ratio of red fluorescence to green fluorescence showing mitochondrial membrane potential after JB treatment. (c) The fluorescent intensity of DCF showing intracellular ROS level after JB treatment. (d) The representative images of intracellular ROS level indicated by DCF fluorescence. Data are presented as the mean ± s.d. of the three independent experiments. *P < 0.05, **P < 0.01 compared with the control group.
