Figure 4
From: Survival of mature T cells depends on signaling through HOIP
Impaired NF-κB and JNK activation in T-HOIPΔlinear T cells.
(a) Isolated CD4+ T cells from HOIP+/+ (blue) or T-HOIPΔlinear (red) mice were stimulated with anti-CD3 mAb followed by anti-hamster IgG for the indicated time. The expression of phospho-IκBα was evaluated by flow cytometry. As the negative control, staining with isotype control IgG was used (filled gray). (b) Isolated CD4+ T cells from HOIP+/+ or T-HOIPΔlinear mice were stimulated with anti-CD3 mAb followed by anti-hamster IgG for the indicated time. The expression of p65 (red) in CD4+ T cells ten min after stimulation were evaluated by confocal microscopy. The nucleus was stained with DAPI (blue). (c) Isolated CD4+ T cells from HOIP+/+ (blue) or T-HOIPΔlinear (red) mice were stimulated with anti-CD3 mAb followed by anti-hamster IgG for the indicated time. The expression levels of phospho-JNK were evaluated by flow cytometry. As the negative control, staining with isotype control IgG was used (filled gray). The data in these figures are representative of four independent experiments.
