Figure 1 | Scientific Reports

Figure 1

From: A Presenilin/Notch1 pathway regulated by miR-375, miR-30a, and miR-34a mediates glucotoxicity induced-pancreatic beta cell apoptosis

Figure 1

Notch1 cleavage was decreased by glucotoxicity.

The mRNA (A) and protein (B) levels were analyzed in INS-1 cells treated for 24 h with different glucose concentrations (mmol/l): 5.5 (control, white bar), 11.1 (black bar), 25 (light grey bar), and 33.3 (heavy grey bar). Quantitative measurements were obtained by gray intensity analysis relative to β-TUBULIN. Relative luciferase activity of the Hes-1 promoter (C) in INS-1 cells treated with 5.5 mmol/l glucose (control) or the indicated glucose concentration. Data shown are means ± SEM and are representative of three separate experiments. *p < 0.05 and **p < 0.01 vs. control.

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