Figure 3

S100A8 immunolocalization in the iris-ciliary body and cornea.

(A–E) Immunofluorescent staining for S100A8 (red) 24 hours after injection of (A) saline; and (B) 18, (C) 24, (D) 36, and (E) 48 hours after injection of LPS into the iris-ciliary body. (F–I) Double labeling of S100A8 (red) and granulocytes (green) at (F) 18, (G) 24, (H) 36, and (I) 48 hours post LPS injection. (J–L) Double labeling of S100A8 (red) and monocytes (green) at (J) 24, (K) 36, and (L) 48 hours after LPS injection. (M–P) Immunofluorescent staining of S100A8 positive cells (red) in the peripheral cornea at (M) 24, (N) 36, and (O) 48 hours post-LPS injection. (P) S100A8 in the central cornea 48 hours after LPS treatment. Bars indicate 50 μm. Results are representative of experiments using 4 separate pairs of eyes in control and LPS-treated groups.