Figure 5
From: Pollen/TLR4 Innate Immunity Signaling Initiates IL-33/ST2/Th2 Pathways in Allergic Inflammation
IL-33 induction by SRW in ocular surface is TLR4-dependent in a topical challenge murine model.
(A,B) BALB/c mice were topically instilled with SRWe at 150 μg/5 μl/eye without or with pre-instilled rat anti-mouse TLR4 antibody (1 μg/5 μl/eye) or its isotype rat IgG2a, and LPS at 5 μg/5 μl/eye or 0.1 μg/5 μl/eye was used as positive control. (C,D) IL-33 induction by topically challenged SRWe in wild type BALB/C and Tlr4-d mice. (E,F) IL-33 induction by topically challenge in MyD88+/+ and MyD88−/− mice. (A,C,E) Show IL-33 mRNA expression by RT-qPCR, and (B,D,F) show its protein levels by ELISA. Results shown are Mean ± SD (n = 5). *P < 0.05, **P < 0.01, compared with PBS controls.
