Figure 6
From: Pollen/TLR4 Innate Immunity Signaling Initiates IL-33/ST2/Th2 Pathways in Allergic Inflammation
IL-33 induction by SRW was TLR4-dependent in HCEC culture model.
(A–C) Primary HCECs were treated with different concentrations of SRWe (1–50 μg/ml) for 0–24 hours to detect IL-33 mRNA and protein production. (D,E) HCECs were pre-incubated with mouse TLR4 antibody (5 μg/ml), isotype mouse IgG2a k, or NF-kB Activation Inhibitor quinazoline (NI, 10 μM) for 1 hour before adding 10 μg/ml SRWe for IL-33 expression at mRNA and protein levels. Results shown are Mean ± SD (n = 5). *P < 0.05, **P < 0.01, compared with untreated cells.
