Table 4 Characteristics of the eight microsatellite loci used for C. glabrata MLVA genotyping.
From: Opportunistic fungal pathogen Candida glabrata circulates between humans and yellow-legged gulls
locus | Chromosome | Gene or intergene | repeat in CBS138 | Forward primer | Reverse primer | Temperature of hybridization |
|---|---|---|---|---|---|---|
GLA2 | I | Intergene | (GCT)8 | 6-FAM-GCACTCTGTCTACTTATAC | CGAATCCGTGATCCCTTC | 54 |
GLA3 | D | Intergene | (AAC)6 | 6-FAM-ACACCTACGAGAAACCAACA | TAGCGGTCATCCAGCATCA | 56 |
GLA4 | A | Intergene | (ATC)8 | VIC-ATGCTGTATTTAACGATGCC | ACCAATGGTAACAGAGT | 54 |
GLA5 | H | CAGL0H02783 g | (ATC)10 | VIC-TTATTACTCTTTCGGGTCAGG | CGAAACAACGTCAGAAACTC | 54 |
GLA6 | J | CAGL0J10560 g | (ATC)9 | VIC-CCTTTAAGGATGAGCTACTTC | GCTGGTGGTTTAAAGGAAAC | 54 |
GLA7 | K | CAGL0K05423 g | (GTT)13 | PET-GTCGTACCTTTGTATAATGTTG | AATGCGTTAGATGCCTT GAGA | 50 |
GLA8 | C | CAGL0C01265 g | (TAA)11 | PET-ATTTACTAATAATACAGCTCAC | CTCAGCAGAACTTTCTT TAGT | 50 |
GLA9 | I | Intergene | (ACTC)5 | PET-CCTTCCCTCTGCGGATACT | TCACTGGACCTCTGTAG TGGT | 56 |
