Figure 2
Variance analysis of metabolites based on HPLC/MS data for rat livers.
(A) A summary of the pathway analysis using MetPA. (B) Plotting PLS-DA scores reveals differences in the metabolic states of the normal control group (red Δ), the 400 μmol/L DMY (high dose) group (green +), the 100 μmol/L DMY (low dose) group (blue ×), the HFD model group (brilliant blue O), and the pioglitazone (positive control) group (purple ∇). PLS-DA score plots of the HPLC/MS data for all groups. (C) A PLS-DA score plot showing the difference in metabolic state between the normal control group (red Δ) and the HFD model group (green +). (D) A PLS-DA score plot showing the difference in the metabolic state between the HFD model group (red Δ) and the pioglitazone (positive control) group (green +). (E) A PLS-DA score plot showing the difference in metabolic state between the 400 μmol/L DMY (high dose) group (red Δ) and the HFD model group (green +). (F) A PLS-DA score plot showing the difference in the metabolic state between the 100 μmol/L DMY (low dose) group (red Δ) and the HFD model group (green +). (G). Heat map visualization of a correlation analysis of all metabolites. Rows: samples; columns: metabolites. The color key indicates the correlation score: blue, lowest; red, highest. n = 6 rats per group. All metabolic pathways are listed in Table S2.
