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Figure 1

From: NPNT is Expressed by Osteoblasts and Mediates Angiogenesis via the Activation of Extracellular Signal-regulated Kinase

Figure 1

Expression patterns of NPNT in osteoblasts.

(A) RT-qPCR was performed during osteoblast differentiation using primers specific for (A) NPNT, (B) ALP, (C) Col1A1, and (D) OCN. Gene expression was normalized to 18S and compared to the expression at day 0. (E) NPNT expression construct (pcDNA3.1-NPNT-c-myc/His), which encodes a mouse full length NPNT, was generated. (F) Detection of NPNT protein in the conditioned medium of COS-7 cells transfected with NPNT expression construct using anti-c-myc antibody. (G) NPNT was detected in osteoblast supernatants using anti-NPNT antibody. HtrA1 was used as positive marker for osteoblast differentiation and β-Actin was used as loading control. Western blot images are presented as cropped format. Full length blots are presented in Supplementary Fig. 4. *P < 0.05, **P < 0.01, ***P < 0.001.

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