Figure 4
NPNT promotes endothelial cell migration, tube-like structure formation and angiogenesis.
(A) Representative microscopic views of scratch wound healing assays performed using SVEC cells treated with recombinant mouse NPNT (500 ng/ml) from 0 to 16 hours. Scale bar, 100 μm. (B) Quantitative analysis of cell migration area. (C) Representative images showing tube-like structure formation by SVEC cells following treatment with recombinant mouse NPNT (500 ng/ml) for 24 hours. Scale bar, 100 μm. (D,E) Quantitative analysis of branch points and tube lengths. PBS and bFGF were used as a negative and positive control respectively. (F) Representative images showing that recombinant mouse NPNT (200 ng/ml) induced vessel outgrowth from metatarsals dissected from E17.5 embryos. Scale bar, 250 μm. (G) Quantitative analysis of vessel sprouting. PBS and VEGF (50 ng/ml) were used as a negative and positive control respectively. *P < 0.05, **P < 0.01, ***P < 0.001.
