Figure 5
NPNT induced phosphorylation of ERK1/2 and p-38.
(A) Western blot images showing the treatment of SVEC cells by mouse recombinant NPNT resulted in the phosphorylation of ERK1/2 and p-38, but not Akt. β-Actin was used as a loading control. (B–D) Quantification of signal intensities of p-ERK1/2, p-p38 and p-Akt by ImageJ. Induction ratios at each timepoint were compared to 0 minute, with p-ERK1/2 normalized to ERK1/2, p-p38 normalized to p-38 and p-Akt normalized to Akt. Western blot images are presented as cropped format. Full length blots are presented in Supplementary Fig. 4. *P < 0.05, **P < 0.01, ***P < 0.001.
