Figure 6 | Scientific Reports

Figure 6

From: NPNT is Expressed by Osteoblasts and Mediates Angiogenesis via the Activation of Extracellular Signal-regulated Kinase

Figure 6

NPNT-induced endothelial cell activities and angiogenesis were inhibited by U0126.

(A) Representative microscopic images of scratch wound healing assays showing NPNT-induced endothelial cell migration was blocked in the presence of U0126 (5 μM). Scale bar, 100 μm. Quantitative analyses showing that (B) NPNT-induced endothelial cell migration and (C) tube-like structure formation were significantly inhibited by U0126. PBS and bFGF were used as a negative and positive control, respectively. (D) Representative images showing that NPNT-induced angiogenesis was inhibited in the presence of U0126 (5 μM). Scale bar, 250 μm. (E) Quantitative analysis of vessel sprouting. *P < 0.05, **P < 0.01, ***P < 0.001.

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