Figure 2
Phosphorylation of Pyk2 is the downstream of Syk signaling.
(A) Immunoblot analysis of p-Pyk2, p-FAK, and p-Syk in PECs from Sykflox/flox/LysM-Cre(−) (wild-type), and Sykflox/flox/LysM-Cre(+) (Syk cKO) mice. Those cells were stimulated with nigericin (left panel) and poly(dA:dT) (right panel) for a period as indicated. The western blot is a representative of three independent experiments. The ratio of p-Pyk2, p-FAK, and p-Syk in the blot were calculated by normalization with total Pyk2, FAK, Syk, respectively, using the values from the unstimulated control cells as 1.0. (B) Immunoblot analysis of p-Pyk2, p-FAK, and p-Syk in PMA-differentiated THP-1 cells that have been pretreated with R406 for 1 h, following stimulation with nigericin (left panel) and poly(dA:dT) (right panel) for a period as indicated. The western blot is a representative of two independent experiments. The ratio of p-Pyk2, p-FAK, and p-Syk in the blot were calculated by normalization with total Pyk2, FAK, Syk, respectively, using the values from the unstimulated control cells as 1.0.
